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Microbiology Specimen Collection and Transfer

Sputum Culture (Routine)

  • Rinse or gargle with water before collection (antiseptic gargle should not be done) – Early morning, coughed up sputum samples are essential (Salivary samples yield normal flora or colonizers only) – Nebulized saline induction, whenever necessary.

AFB Culture – 3 consecutive early morning sputum samples in adults (Can be refrigerated and transported together) – In neonates and small children - gastric aspirate may be cultured.

Disclaimer: This guide is an educational reference for healthcare professionals. It does not replace clinical judgement — always follow your hospital's protocols and current guidelines.