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Microbiology Specimen Collection and Transfer

Urine Culture (Routine)

  • Mid stream urine sample is collected into sterile container after genital toilet. – If on indwelling catheter, aspirate from the proximal end after disinfecting the area (never collect from urobag) (Culture of Foleys catheter tip is not useful).

Suprapubic Aspiration – For infants and children below 6 years. – Also when anaerobic cultures are required.

Urine – AFB Culture – 3 consecutive early morning whole urine samples in a large sterile container (Urine may be refrigerated and transported on the third day to the laboratory).

Disclaimer: This guide is an educational reference for healthcare professionals. It does not replace clinical judgement — always follow your hospital's protocols and current guidelines.